rabbit anti importin β1 antibody  (Proteintech)

Journal: bioRxiv

Article Title: Two sequential waves of mRNA translation drive embryonic development

doi: 10.1101/2025.09.18.676998

Figure Lengend Snippet: a Double staining of GFP-Ewsr1b (green) and GFP-Ewsr1b mRNA (red) in embryos injected with GFP-Ewsr1b mRNA carrying Long-3′UTR (Long) or Short-3′UTR (Short) at 4 hpf. Scale bars: 20 µm. b Violin plots showing distances from the nuclear center to signals of GFP-Ewsr1b mRNA carrying Long-3′UTR or Short-3′UTR (means ± SD; n = 80). Similar results were obtained from two independent experiments. **********p < 0.0000000001 (Student’s t -test). c Immunofluorescence of Importin β1 in embryos at 3 hpf. d Immunoblotting of embryos at 3 hpf following IP with control IgG (IgG) or anti-Importin β1 (α-Im β) antibody, and RT-PCR for ewsr1b -3′Long and α-tubulin mRNAs. Similar results were obtained from two independent experiments. e Double staining of Importin β1 (red) and the ewsr1b -3′Long mRNA 3′UTR (green) in embryos at 3 hpf. Left: High-resolution confocal image; right: enlarged views of the boxed area. Similar results were obtained from two independent experiments. f Immunofluorescence of Importin β1 and Ewsr1b in uninjected embryos (Control) or embryos injected with Importazole at 3 hpf. DNA is shown in blue. Enlarged views of the boxed area with or without DNA staining are shown on the right side. Scale bars, 10 µm. g Quantification of average signal intensity in the nucleus per 25 µm 2 . (means ± SD; n = 10). ***********p < 0.00000000001 (Student’s t -test).

Article Snippet: Proteins were separated by SDS-PAGE, transferred onto Immobilon membranes, and probed with primary antibodies; mouse anti-Syncrip antibody (1:1,000, hnRNP Q; Santa Cruz Biotechnology, I8E4; sc-56703), rabbit anti-Syncrip antibody (1:1,000, Proteintech, 14024-1-AP), rabbit anti-Rpl11 antibody (1:1,000, Abcam, ab79352), rabbit anti-Pou5f3 antibody (1:100), mouse anti-GFP antibody (1:1,000, Roche, 11814460001), mouse anti-Ewsr1b antibody (1:100, present study), rabbit anti-Importin β1 antibody (1:1,000, Proteintech, 10077-1-AP), and mouse anti-HuR antibody (1:1000, Santa Cruz Biotechnology; sc-5261).

Techniques: Double Staining, Injection, Immunofluorescence, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: Pharmaceuticals

Article Title: Jietacin Derivative Inhibits TNF-α-Mediated Inflammatory Cytokines Production via Suppression of the NF-κB Pathway in Synovial Cells

doi: 10.3390/ph16010005

Figure Lengend Snippet: Effect of jietacin derivative on the NF-κB pathway in SW982. Western blotting for p65, phosphorylated p65 (p-p65), importin α3, importin β1 and GAPDH ( A ). Densitometry of western blot protein bands for p-p65 ( B ), p65 ( C ), importin α3 ( D ), and importin β1 ( E ) were normalized to the expression of GAPDH ( n = 3). Vehicle, Lane 1,6,11; TNF-α, Lane 2,7,12; TNF-α+ 2.5 JD, Lane 3,8,13; TNF-α+ 1.25 JD, Lane 4,9,14; TNF-α+ 0.625 JD, Lane 5,10,15. a p < 0.05 in comparison with vehicle, b p < 0.05 compared to TNF-α.

Article Snippet: It was then incubated with anti-p65 mouse monoclonal antibody (1:10,000; cat no. #6956, Cell Signaling Technology, Boston, MA, USA), anti-phospho-p65 (Ser536) rabbit monoclonal antibody (1:10,000; cat no. #76778, Cell Signaling Technology), anti-importin α3 (affinity-purified rabbit polyclonal importin α3 antibody was raised against a synthetic peptide (C)GFNSSTNVPTEGFQF corresponding to the mouse importin α3 C-terminal region), anti-importin β1 rabbit monoclonal antibody (1:1000; cat no. #51186), anti-p38 MAPK (1:1000; cat no. #9212, Cell Signaling Technology), anti-phospho-p38 MAPK (Thr180/Tyr182) (1:1000; cat no. 9211, Cell Signaling Technology), or anti-GAPDH antibody (1:5000; FUJIFILM Wako Pure Chemical Co., Osaka, Japan) at 25 °C for 60 min.

Techniques: Western Blot, Expressing, Comparison

Journal: Pharmaceuticals

Article Title: Jietacin Derivative Inhibits TNF-α-Mediated Inflammatory Cytokines Production via Suppression of the NF-κB Pathway in Synovial Cells

doi: 10.3390/ph16010005

Figure Lengend Snippet: Effect of jietacin derivative on the NF-κB pathway in human primary synovial fibroblasts. Western blotting for p65, phosphorylated p65 (p-p65), importin α3, importin β1, and GAPDH ( A ). Densitometry of Western blot protein bands for p-p65 ( B ), p65 ( C ), importin α3 ( D ), and importin β1 ( E ) were normalized to the expression of GAPDH ( n = 3). Vehicle, Lane 1,4,7; TNF-α, Lane 2,5,8; TNF-α+ 2.5 JD Lane 3,6,9. a p < 0.05 compared with vehicle, b p < 0.05 compared to TNF-α.

Article Snippet: It was then incubated with anti-p65 mouse monoclonal antibody (1:10,000; cat no. #6956, Cell Signaling Technology, Boston, MA, USA), anti-phospho-p65 (Ser536) rabbit monoclonal antibody (1:10,000; cat no. #76778, Cell Signaling Technology), anti-importin α3 (affinity-purified rabbit polyclonal importin α3 antibody was raised against a synthetic peptide (C)GFNSSTNVPTEGFQF corresponding to the mouse importin α3 C-terminal region), anti-importin β1 rabbit monoclonal antibody (1:1000; cat no. #51186), anti-p38 MAPK (1:1000; cat no. #9212, Cell Signaling Technology), anti-phospho-p38 MAPK (Thr180/Tyr182) (1:1000; cat no. 9211, Cell Signaling Technology), or anti-GAPDH antibody (1:5000; FUJIFILM Wako Pure Chemical Co., Osaka, Japan) at 25 °C for 60 min.

Techniques: Western Blot, Expressing